Our Research Most mental illnesses begin during adolescence and 1 in 4 youth meet criteria for a psychiatric disorder. Yet
the biological causes for this heightened vulnerability are unknown and there are no biomarkers to detect youth at high risk. Our research aims at understanding the neurobiology of psychiatric vulnerability in adolescence and discovering biomarkers of risk and resilience during this age. We study neurobiological processes ongoing during adolescent brain development and how these events are modulated by exposure to risk or protective factors. We apply multidisciplinary and translational approaches to identify key genes controlling the adolescent maturation of the prefrontal cortex in males and females. Using molecular
neuroanatomical
neurochemical
pharmacological
genetic
and behavioral tools
we have demonstrated that a set of guidance cue genes and their microRNA regulators orchestrate the development of the prefrontal cortex in adolescence
determine differential vulnerability to develop psychopathology-like phenotypes in mice and are tightly associated with substance abuse
schizophrenia and major depression in humans. Skills & Qualifications • Ability to work independently and efficiently. • Strong organizational and task prioritization skills. • Excellent communication skills and proficiency in performing administrative and clerical tasks. • Proficient in general laboratory procedures
techniques
and documentation. • Willingness to learn and adapt to new techniques and technologies. • Fluent in English
Spanish
French
and Catalan. • Proficient in statistical analysis and software such as SPSS
MATLAB
and Python. • Proficient in using various software programs
including Microsoft Office Suite (Word
Excel
PowerPoint). • Advanced knowledge and experience in 3D cell culture techniques. • Skilled in protein isolation
Western Blot
PCR
rt-qPCR
toxicity testing
IHC
Northern Blot
and ELISA. • Proficient in anatomical dissection studies for medical and veterinary purposes. • Experienced in static analysis of behavioral data and microarray data. • Familiarity with electrophysiology
imaging
protein purification
and optical and electron microscopy techniques. • Advanced level proficiency in conducting animal experiments
behavioral experiments
anatomical dissection
and molecular analysis. Guidance cues and stress-induced prefrontal cortex plasticity This line of research is concerned with characterizing the cellular and molecular mechanisms and the specific neuronal pathways mediating the role of the Netrin-1/DCC pathway in the development of susceptibility/resilience to the effects of chronic stress in adulthood. Our goal is to understand how adult aversive experiences disrupt the organization of prefrontal cortex circuitries
rendering some
but not all individuals vulnerable to develop maladaptive behaviors. Discovering biomarkers of psychiatric risk in adolescence microRNAs as mediator and biomarkers of psychiatric vulnerability The identification of early markers of psychiatric risk is urgently needed. This translational line of work examines the role of microRNAs in the development of the prefrontal cortex in adolescence and in individual differences in vulnerability to mental illness. microRNAs can be measured non-invasively in peripheral samples and could serve as longitudinal readouts of brain expression and psychiatric risk in youth. * Figure taken from Morgunova & Flores (2021) Seminars in Cell and Developmental Biology Genetic profiling (PRS) In collaboration with Drs. Patricia Pelufo Silveira and Michael Meaney we are beginning to use novel approaches to genomic profiling
that are informed by biological function and characterize gene networks
to assess psychiatric risk in adolescence Understanding how experiences in adolescence shape the developing brain Drugs of Abuse Drug use during adolescence is a strong predictor of lifetime abuse. This line of work seeks to establish how stimulant drugs affect the developmental changes that are ongoing in the adolescent brain and their behavioral consequences. The work is carried out in both males and females and examines effects of excessive drug exposure and therapeutic-like doses as well as critical periods of vulnerability within adolescence. We also examine the effects of other types of drugs
including cannabinoids and opioids. Created with BioRender.com Stressors Exposure to social stress during adolescence increases vulnerability to lifetime cognitive deficits associated with psychiatric disorders
but not all adolescents are equally susceptible. This line of research examines how exposure to social defeat stress in adolescence alters the adolescent developing brain and the maturation of cognitive processing. We are particularly interested in discovering mechanisms and biomarkers of susceptibility and resilience to these effects. We carry carry out this work in both males and females Position: Research Assistant- Full Time - Molecular and/or Neuroanatomy methods Research assistant-full time - molecular and/or neuroanatomy methods Summary of position: The laboratory of Dr. Cecilia Flores (lab website) at McGill University is looking for a full time Research Assistant with experience in molecular biology methods and/or neuroanatomy to join her dynamic translational research group Description of tasks: Perform molecular biology and/or neuroanatomy experiments Perform basic lab experiments Required qualifications: Bachelor degree or equivalent Required skills: Experience working with quantitative molecular biology and/or neuroanatomy methods Excellent organizational skills Required abilities: Able to work independently and take initiative Good interpersonal communication skills Location: Douglas Research Center Work Schedule: Full-time (Monday-Friday
35 hours/week) Instructions for Submitting Application: To apply
please send your resume
and cover letter to sehar.gul2@mail.mcgill.ca and Cc Dr. Cecilia Flores at cecilia.flores@mcgill.ca Position: Research Associate- Proficient in Immunohistochemistry and Stereology Dr. Cecilia Flores' laboratory is looking for a research associate or research assistant who has skills and expertise in neuroanatomy and immunohistochemistry (IHC). The candidate should also have proficiency in wet laboratory methods and techniques and good understanding in the analysis of biological samples. Tasks: -Conduct immunohistochemistry and imaging analysis -Perform tissue sample processing and cryosection for histological analysis. -Optimize and execute IHC protocols
including antigen retrieval
blocking
antibody incubation
signal detection
and visualization. -Employ stereological principles to perform unbiased sampling and quantification of three-dimensional biological structures. -Operate microscopes and imaging systems for acquiring high-resolution images and conduct image analysis using appropriate software. -Analyze experimental data
generate statistical reports
and contribute to the interpretation and presentation of research findings. -Assist in training junior laboratory personnel on experimental techniques and safety procedures. Required Qualifications and Skills: Education: Master of Science (M.Sc.) or relevant demonstrated laboratory experience Immunohistochemistry (IHC): Proficient in IHC procedures
including tissue sample processing
antigen retrieval
blocking
primary antibody incubation
signal detection
and visualization techniques. Neuroanatomical analysis: Knowledge of stereological principles and methodologies
including tissue sectioning
probe design
unbiased sampling
stereological calculations
and estimation of three-dimensional biological structures. Laboratory Techniques: Skilled in histological sample preparation
cryosection
microtome operation
immunofluorescence staining
microscopy
image analysis
and data interpretation. Research Methodologies: Experienced in designing and executing experiments
data collection
troubleshooting technical issues
and maintaining accurate experimental records. Data Analysis: Experience using statistical software and image analysis tools to analyze and interpret experimental data
generate graphs
and prepare scientific reports and presentations. Quality Control: Strong adherence to quality control procedures
ensuring accuracy
reproducibility
and consistency of experimental results. Team Collaboration: Excellent interpersonal skills
fostering effective communication and collaboration with team members
researchers
and laboratory staff. Laboratory Safety: Committed to maintaining a safe and clean laboratory environment
following standard operating procedures (SOPs)
and ensuring compliance with safety regulations. Required Abilities: -Excellent interpersonal
written
and verbal communication skills -Ability to prioritize multiple tasks and meet deadlines -Strong attention to detail and organizational skills -Capacity to work in a multidisciplinary team in a fast-paced
result-oriented environment -Legally able to work in Canada Location: Douglas Research Center Work Schedule: Full-time (Monday-Friday
35 hours/week) Instructions for Submitting Application: To apply
please send your resume
cover letter
and any relevant publications/GitHub projects to sehar.gul2@mail.mcgill.ca and Cc Dr. Cecilia Flores at cecilia.flores@mcgill.ca