De Strooper & Arancibia Lab Cellular Phase Of Alzheimer’s Disease Laboratory Cover Letter

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de strooper & arancibia lab cellular phase of alzheimer’s disease laboratory Cover Letter
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Research assistant  At de Strooper & Arancibia lab Cellular Phase of Alzheimer’s Disease Laboratory Cover Letter

Research assistant Cover Letter At de Strooper & Arancibia lab Cellular Phase of Alzheimer’s Disease Laboratory

de Strooper & Arancibia lab Cellular Phase of Alzheimer’s Disease Laboratory

USKOKMER- Stem Cells Research Center Istanbul
Turkey Uskudar University November2019 -August 2022 Principal Investigator: Sevim Isık
Ph.D. Research Project Coordinator/ Graduate Researcher • Participated in the writing of the TÜBİTAK 1001 219S842 project "Investigation of the Role of DNA Topoisomerase IIβ in Neuroinflammation in the A53T α-Synuclein Mutant Transgenic Mouse Model of Parkinson's Disease and In Vitro Illumination of Related Molecular Pathways." • Wrote the project "Testing hydrogel-supported stem cell therapies in traumatic brain injuries in a mouse model" within the scope of the research fund of the Presidency of Health Institutions of Turkey (TUSEB)
supporting stem cell applications in neurological diseases. • Wrote the project "Treatment of deformed glandular parotic with stem cells in oncology patients receiving chemotherapy and radiotherapy" within the scope of the bilateral cooperation of Turkish-Korean science institutes. • Wrote the project "Investigation of inflammatory mechanisms thought to cause schizophrenia in the ACE2 TG transgenic mouse model" funded by the Turkish Science and Technical Research Council (TUBITAK) for innovative treatments in neuropsychiatric diseases. • Completed a master's thesis titled "Examination of Topo IIß Gene Expression in LPS-Induced HMC3 Microglia Cell Line" as a project parameter for the TÜBİTAK 1001219S842 project in genetics and neuroinflammation studies. Conducted optimization studies of microglia (HMC3) cell lines under cell culture conditions. Sequenced and optimized the topoisomerase IIß gene by obtaining a plasmid. Explored transfection reagents for overexpression and induction of the gene. Created a neuroinflammation model in HMC3 cell culture and analyzed its efficiency with TNF- alpha
LPS
and ATP catalysts. Employed microscopy and flow cytometry methods to observe changes in microglia morphology and phenotype differences. Confirmed the obtained data through RNA (rt-qPCR) and protein level (Western Blot) techniques. • Established a neuroinflammation model by co-culturing neuron (SHSY5Y neuroblastoma cell line)
astrocyte (C6 glioma cell line)
and microglia (HMC3 cell line) cell lines to study crosstalk between cells in neuroinflammation. Investigated the effect of the topoisomerase IIß gene on neuroinflammation patterns. Utilized flow cytometry
protein level (Western Blot)
and RNA level (rt-qPCR) methods for model confirmation. • Induced cells with retinoic acid (RA) and Human brain-derived neurotrophic factor (BDNF) for differentiation. Monitored the differentiation process using a real-time cell analysis system. Confirmed neurite and axon elongation through immunohistochemistry methods. Constructed a Parkinson's model using MPP+ after neural differentiation. Employed the immunofluorescence method to establish the Parkinson's model. Controlled the expression of the topoIIß gene in the Parkinson's model at protein (Western Blot) and RNA (rt-qPCR) levels. • Organized the journal club on stem cell and regenerative medicine methods in the treatment of neurodegenerative diseases on a weekly basis. Coordinated laboratory colleagues in presenting new developments from the relevant literature. Skills & Qualifications • Ability to work independently and efficiently. • Strong organizational and task prioritization skills. • Excellent communication skills and proficiency in performing administrative and clerical tasks. • Proficient in general laboratory procedures
techniques
and documentation. • Willingness to learn and adapt to new techniques and technologies. • Fluent in English
Spanish
French
and Catalan. • Proficient in statistical analysis and software such as SPSS
MATLAB
and Python. • Proficient in using various software programs
including Microsoft Office Suite (Word
Excel
PowerPoint). • Advanced knowledge and experience in 3D cell culture techniques. • Skilled in protein isolation
Western Blot
PCR
rt-qPCR
toxicity testing
IHC
Northern Blot
and ELISA. • Proficient in anatomical dissection studies for medical and veterinary purposes. • Experienced in static analysis of behavioral data and microarray data. • Familiarity with electrophysiology
imaging
protein purification
and optical and electron microscopy techniques. • Advanced level proficiency in conducting animal experiments
behavioral experiments
anatomical dissection
and molecular analysis.de Strooper & Arancibia lab Cellular Phase of Alzheimer’s Disease Laboratory Lab content In this section Overview Vacancies Publications Lab members Artistic representation of spatial transcriptomic data around amyloid plaques. Artistic representation of spatial transcriptomic data around amyloid plaques. Created by Duygu Koldere Vilain.TOGGLE TO VIEW HERO CAPTION Contact Bart De Strooper Job title:Principal Group Leader Lorena Arancibia Carcamo Job title:Group Member - Secondment Related links UK Dementia Research Institute Related topics BIOCHEMISTRY & PROTEOMICS CELL BIOLOGY COMPUTATIONAL & SYSTEMS BIOLOGY HUMAN BIOLOGY & PHYSIOLOGY MODEL ORGANISMS NEUROSCIENCES STEM CELLS Alzheimer’s Disease causes hundreds of interconnected changes in the brain many years before the onset of clinical symptoms. We want to understand the mechanisms behind these changes and use this information to identify potential new treatments. Alzheimer’s disease (AD) is characterised by the abnormal build-up of protein in the brain. Most researchers believe that these protein clusters
or the mechanisms that form them
may be important in the progress of the disease. One of these proteins
Amyloid beta (Aβ) is produced from the breakdown of Amyloid Precursor Protein and historically Alzheimer’s research has focused on this chain reaction as a possible target for new treatments. However
most clinical trials attempting to target this pathway have been unsuccessful. In our lab
we are looking at the effects of Aβ on the immune and support cells of the brain and how this changes how cells interact with each other. We combine studies of individual brain cells and cell networks with genetic screening to identify mechanisms behind the disease that could lead to the development of new treatments.

By Melike Cansel EREN


Dear Bart De Strooper and Lorena Arancibia Carcamo, Greetings from your potential future Research Assistant, Melike Cansel EREN! I came across the open position for a Research Assistant at your esteemed company, de Strooper & Arancibia lab Cellular Phase of Alzheimer’s Disease Laboratory, and I couldn't resist the urge to apply. Why, you may ask? Well, let me tell you, I have been a fan of your work for quite some time now. Firstly, let me introduce myself. I am a researcher with a background in stem cell research, specifically in the field of neuroinflammation and genetics. My passion for neuroscience and the mysteries of the brain has led me on a journey to explore different aspects of neurodegenerative diseases. And what better place to continue this journey than at your lab? I must say, I was quite impressed by the extensive list of projects you have undertaken, ranging from investigating the role of DNA topoisomerase IIβ in neuroinflammation in Parkinson's disease to exploring stem cell therapies for traumatic brain injuries. It's like a dream come true for a neuroscientist like me. Moreover, I was intrigued by your use of cutting-edge technologies, such as spatial transcriptomics and genetic screening, to unravel the complexities of Alzheimer's disease. As someone who is always eager to learn and adapt to new techniques, I am delighted at the opportunity to be a part of such innovative research. But what truly caught my attention was your lab's focus on understanding the mechanisms behind the hundreds of interconnected changes in the brain that occur in Alzheimer's disease. As we all know, this disease is a puzzle that still needs to be solved, and I believe that your lab's approach is a step in the right direction. I am excited to be a part of this journey towards finding potential new treatments for Alzheimer's. Now, let's talk about my skills. I must say, I am quite a catch when it comes to research. My previous experiences have honed my abilities to work independently and efficiently. I have strong organizational and task prioritization skills, which have helped me manage multiple projects simultaneously. And let's not forget my excellent communication skills, which have come in handy when presenting my research findings and coordinating with my colleagues. I am also proficient in performing administrative and clerical tasks, making me a well-rounded researcher. My knowledge of general laboratory procedures, techniques, and documentation will surely be an asset to your team. And if there's one thing that is certain, it's my willingness to learn and adapt to new techniques and technologies. In addition to my strong research background, I am fluent in English, Spanish, French, and Catalan. My proficiency in these languages has allowed me to work and communicate effectively with researchers from different parts of the world. I am also skilled in statistical analysis and software such as SPSS, MATLAB, and Python, making me a valuable asset for data analysis in your lab. My experience in 3D cell culture techniques, protein isolation, Western Blot, PCR, rt-qPCR, toxicity testing, IHC, Northern Blot, and ELISA will surely come in handy in your lab, where such techniques are used extensively. I am also well-versed in conducting animal experiments, behavioral experiments, anatomical dissection, and molecular analysis, making me a perfect fit for your team. Lastly, I must mention my love for learning. I am always on the lookout for new developments and advancements in the field of stem cell and regenerative medicine. In fact, I have organized a journal club on these topics in the past, where I coordinated my colleagues in presenting new developments from the relevant literature. I believe this thirst for knowledge and my passion for research align perfectly with the values of your lab. In conclusion, I am beyond excited at the prospect of becoming a part of your team. I am confident that my skills, passion, and love for learning will make me a valuable addition to your lab. Thank you for considering my application, and I look forward to discussing this opportunity further. Sincerely, Melike Cansel EREN.