Are you interested in studying the blood-brain barrier and how inflammation influences brain function? We are seeking a research assistant in Chenghua Gu’s laboratory at Harvard Medical School. Within the Gu lab
the neuroimmunology subgroup studies immune signaling at the blood-brain barrier during infection and inflammation. We seek to understand the basic mechanisms underlying blood-brain barrier permeability
immune cell recruitment
and communication networks with other cell population in the brain. The research assistant will work with other lab members to study these immune signaling networks at the blood-brain barrier using a variety of imaging strategies
mouse genetics
immunophenotyping
as well as other cell and molecular biology techniques. The team consists of PhD and MD-PhD trainees and post-docs at all levels. The ideal research assistant will be well-organized
self-motivated
and demonstrate responsibility. While here
the individual will gain experience with the scientific method
experimental design
leadership
and time management
and have the freedom to implement their own ideas to help to accelerate scientific projects. Additionally
there are many opportunities for career development and academic growth within the Gu lab and the larger Harvard Medical School system. On day-to-day basis
the individual will work on experiments employing the following methods: Molecular biology (plasmid cloning
gene editing
viral construct building etc.) Biochemistry (western blot
ELISA). Tissue culture (transfection
viral infection
primary cell culture). Histology (immunostaining
EM
tissue clearance
etc.)
Imaging and imaging analysis (confocal
super resolution
slidescanner
etc) and cell profiling and other imaging tools for analysis. Cell sorting including flow cytometry
FACS
and MACS. Mouse genetics and animal models of disease and inflammation.